Browse Text search Protein search Domain search Download User manual Related links Citation & Contact

The OmpW Family [Function: Specific diffusion channels] Seed alignment | Full alignment | Pfam page | Pfam Wiki page | TC-DB page

This family consists of homologs to OmpW protein of Salmonella typhimurium, Escherichia coli and Vibrio cholerae (minor outer membrane proteins) and the major outer membrane protein OprG of Pseudomonas aeruginosa. OprG appears to be involved in low-affinity iron uptake and transport hydrophobic compounds by using a lateral gate in the barrel wall thought to be lined by three conserved prolines, whereas OmpW is also the Receptor for colicin S4. AlkL, a minimalistic outer-membrane protein from oil-consuming bacteria, exploits dynamics of extracellular loops to channel substrate across the polysaccharide barrier and into the hydrophobic interior of the outer membrane. Homologs have been found in many Gram-negative bacterial genera including Azotobacter, Desulfitobacterium, Burkholderia, Xanthomonas, Rhodobacter, Ralstonia, Delftia and Rickettsia. Proteins of the family show low sequence similarity to members of the OprF family, while the structures of both OmpW of Escherichia coli and OprG from Pseudomonas aeruginosa, revealed an 8-stranded ß-barrel.

Specify pHMMs' % coverageUnspecified More than   
Representative image:  fam_img

Literature references

Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR
Biomol NMR Assign. 2020 Oct;14(2):295-300. doi: 10.1007/s12104-020-09964-5. Epub 2020 Jun 30.
PMID: 32607893

Designing an Outer Membrane Protein (Omp-W) Based Vaccine for Immunization against Vibrio and Salmonella: An in silico Approach
Recent Pat Biotechnol. 2020;14(4):312-324. doi: 10.2174/1874609813666200929113341.
PMID: 32990553

Quaternary structure of the small amino acid transporter OprG from Pseudomonas aeruginosa
J Biol Chem. 2018 Nov 2;293(44):17267-17277. doi: 10.1074/jbc.RA118.004461. Epub 2018 Sep 20.
PMID: 30237175

Discovery of novel inhibitors of multidrug-resistant Acinetobacter baumannii
Bioorg Med Chem. 2017 Oct 15;25(20):5477-5482. doi: 10.1016/j.bmc.2017.08.014. Epub 2017 Aug 9.
PMID: 28830719

Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H
Microb Pathog. 2016 Sep;98:57-62. doi: 10.1016/j.micpath.2016.06.024. Epub 2016 Jun 28.
PMID: 27364548

OprG Harnesses the Dynamics of its Extracellular Loops to Transport Small Amino Acids across the Outer Membrane of Pseudomonas aeruginosa
Structure. 2015 Dec 1;23(12):2234-2245. doi: 10.1016/j.str.2015.10.009. Epub 2015 Nov 19.
PMID: 26655471

Optimizing nanodiscs and bicelles for solution NMR studies of two ß-barrel membrane proteins
J Biomol NMR. 2015 Apr;61(3-4):261-74. doi: 10.1007/s10858-015-9905-z. Epub 2015 Feb 10.
PMID: 25869397

NMR polypeptide backbone conformation of the E. coli outer membrane protein W
Structure. 2014 Aug 5;22(8):1204-1209. doi: 10.1016/j.str.2014.05.016. Epub 2014 Jul 10.
PMID: 25017731

Expression of the Escherichia coli ompW colicin S4 receptor gene is regulated by temperature and modulated by the H-NS and StpA nucleoid-associated proteins
FEMS Microbiol Lett. 2014 Mar;352(2):238-44. doi: 10.1111/1574-6968.12385. Epub 2014 Jan 31.
PMID: 24444297

Outer membrane protein OmpW of Escherichia coli is required for resistance to phagocytosis
Res Microbiol. 2013 Oct;164(8):848-55. doi: 10.1016/j.resmic.2013.06.008. Epub 2013 Jun 25.
PMID: 23811183

Eight stranded ß -barrel and related outer membrane proteins: role in bacterial pathogenesis
Protein Pept Lett. 2012 Oct;19(10):1013-25. doi: 10.2174/092986612802762688.
PMID: 22533621

Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles
J Biomol NMR. 2012 Oct;54(2):129-33. doi: 10.1007/s10858-012-9658-x. Epub 2012 Aug 14.
PMID: 22890565

The crystal structure of OprG from Pseudomonas aeruginosa, a potential channel for transport of hydrophobic molecules across the outer membrane
PLoS One. 2010 Nov 29;5(11):e15016. doi: 10.1371/journal.pone.0015016.
PMID: 21124774

Identification of outer membrane proteins with emulsifying activity by prediction of beta-barrel regions
J Microbiol Methods. 2009 Jan;76(1):52-7. doi: 10.1016/j.mimet.2008.09.012. Epub 2008 Sep 23.
PMID: 18848588

The outer membrane protein OmpW forms an eight-stranded beta-barrel with a hydrophobic channel
J Biol Chem. 2006 Mar 17;281(11):7568-77. doi: 10.1074/jbc.M512365200. Epub 2006 Jan 12.
PMID: 16414958

Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membrane
J Bacteriol. 1999 Jun;181(11):3578-81. doi: 10.1128/JB.181.11.3578-3581.1999.
PMID: 10348872

Identification of oprG, a gene encoding a major outer membrane protein of Pseudomonas aeruginosa
J Antimicrob Chemother. 1999 Apr;43(4):607-8. doi: 10.1093/jac/43.4.607.
PMID: 10350397

Proteins in this family with 3D-structure
View Entry
# strands