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The DUF2219 (SfpA/LpxR) Family [Function: Enzyme] Seed alignment | Full alignment | Pfam page

This family consists of several proteins found in various pathogenic bacteria. The prototype of the family, SfpA of Yersinia enterocolitica, is heat modifiable, which has been detected in the membrane fraction after fractionization. Analysis of bacterial colonization revealed that a {Delta} SfpA strain is defective in mesenteric lymph node colonization. A very close homolog, has been designated as the lipid A-3-O deacylase (LpxR) that has been identified in the outer membrane of Salmonella typhimurium. Its strucure has been solved and suggests a ß-barrel of 12 ß strands.

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Literature references

Helicobacter pylori Resists the Antimicrobial Activity of Calprotectin via Lipid A Modification and Associated Biofilm Formation
mBio. 2015 Dec 8;6(6):e01349-15. doi: 10.1128/mBio.01349-15.
PMID: 26646009

Lipid A 3'-O-deacylation by Salmonella outer membrane enzyme LpxR modulates the ability of lipid A to stimulate Toll-like receptor 4
Biochem Biophys Res Commun. 2012 Nov 23;428(3):343-7. doi: 10.1016/j.bbrc.2012.10.054. Epub 2012 Oct 22.
PMID: 23085233

Phosphate groups of lipid A are essential for Salmonella enterica serovar Typhimurium virulence and affect innate and adaptive immunity
Infect Immun. 2012 Sep;80(9):3215-24. doi: 10.1128/IAI.00123-12. Epub 2012 Jul 2.
PMID: 22753374

Palmitoylation state impacts induction of innate and acquired immunity by the Salmonella enterica serovar typhimurium msbB mutant
Infect Immun. 2011 Dec;79(12):5027-38. doi: 10.1128/IAI.05524-11. Epub 2011 Sep 19.
PMID: 21930761

Active-site architecture and catalytic mechanism of the lipid A deacylase LpxR of Salmonella typhimurium
Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1960-4. doi: 10.1073/pnas.0813064106. Epub 2009 Jan 27.
PMID: 19174515

Structural biology of membrane-intrinsic beta-barrel enzymes: sentinels of the bacterial outer membrane
Biochim Biophys Acta. 2008 Sep;1778(9):1881-96. doi: 10.1016/j.bbamem.2007.07.021. Epub 2007 Aug 11.
PMID: 17880914

Characterization of a novel porin involved in systemic Yersinia enterocolitica infection
Infect Immun. 2006 Jul;74(7):4361-5. doi: 10.1128/IAI.00154-06.
PMID: 16790812

An outer membrane enzyme encoded by Salmonella typhimurium lpxR that removes the 3'-acyloxyacyl moiety of lipid A
J Biol Chem. 2006 Aug 4;281(31):21974-21987. doi: 10.1074/jbc.M603527200. Epub 2006 May 16.
PMID: 16704973

Proteins in this family with 3D-structure
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