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The Borrelia burgdorferi attachment protein P66 Family [Function: Non-specific diffusion channels] Seed alignment | Full alignment | Pfam page | TC-DB page

Members of this family (formerly, The Borrelia Oms66/Omp66 Family)are found in many Borrelia species. They are large outer membrane proteins that exhibit porin activity in planar lipid bilayers. The average single-channel conductance predicts a rather large pore diameter of 2.6 nm. The exact number of transmembrane strands is unknown but prediction methods suggest a large number, which is in agreement with the large pore. P66 may in fact form a 2022-stranded ß-barrel structure. There is evidence that the C-terminal region may traverse through the channel and be surface-accessible. There are also indications that native P66 can elicit potent bactericidal activity and significant protective immunity against host-adapted organisms.

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Literature references

HtrA of Borrelia burgdorferi Leads to Decreased Swarm Motility and Decreased Production of Pyruvate
mBio. 2018 Jul 10;9(4):e01136-18. doi: 10.1128/mBio.01136-18.
PMID: 29991588

Intravital Imaging of Vascular Transmigration by the Lyme Spirochete: Requirement for the Integrin Binding Residues of the B. burgdorferi P66 Protein
PLoS Pathog. 2015 Dec 18;11(12):e1005333. doi: 10.1371/journal.ppat.1005333. eCollection 2015 Dec.
PMID: 26684456

Borrelia burgdorferi?HtrA: evidence for twofold proteolysis of outer membrane protein p66
Mol Microbiol. 2016 Jan;99(1):135-50. doi: 10.1111/mmi.13221. Epub 2015 Oct 20.
PMID: 26370492

Cross-Reactive Epitopes in Borrelia burgdorferi p66
Clin Vaccine Immunol. 2015 Jul;22(7):840-3. doi: 10.1128/CVI.00217-15. Epub 2015 May 13.
PMID: 25972406

Multilocus spacer analysis revealed highly homogeneous genetic background of Asian type of Borrelia miyamotoi
Infect Genet Evol. 2015 Apr;31:257-62. doi: 10.1016/j.meegid.2015.02.009. Epub 2015 Feb 16.
PMID: 25697887

Structural modeling and physicochemical characterization provide evidence that P66 forms a ß-barrel in the Borrelia burgdorferi outer membrane
J Bacteriol. 2014 Feb;196(4):859-72. doi: 10.1128/JB.01236-13. Epub 2013 Dec 6.
PMID: 24317399

Use of nonelectrolytes reveals the channel size and oligomeric constitution of the Borrelia burgdorferi P66 porin
PLoS One. 2013 Nov 6;8(11):e78272. doi: 10.1371/journal.pone.0078272. eCollection 2013.
PMID: 24223145

The role of Borrelia burgdorferi outer surface proteins
FEMS Immunol Med Microbiol. 2012 Oct;66(1):1-19. doi: 10.1111/j.1574-695X.2012.00980.x. Epub 2012 May 21.
PMID: 22540535

The Borrelia burgdorferi integrin ligand P66 affects gene expression by human cells in culture
Infect Immun. 2011 Aug;79(8):3249-61. doi: 10.1128/IAI.05122-11. Epub 2011 May 16.
PMID: 21576330

P66 porins are present in both Lyme disease and relapsing fever spirochetes: a comparison of the biophysical properties of P66 porins from six Borrelia species
Biochim Biophys Acta. 2010 Jun;1798(6):1197-203. doi: 10.1016/j.bbamem.2010.02.011. Epub 2010 Feb 25.
PMID: 20188698

Protection elicited by native outer membrane protein Oms66 (p66) against host-adapted Borrelia burgdorferi: conformational nature of bactericidal epitopes
Infect Immun. 2000 May;68(5):2647-54. doi: 10.1128/iai.68.5.2647-2654.2000.
PMID: 10768956

A surface-exposed region of a novel outer membrane protein (P66) of Borrelia spp. is variable in size and sequence
J Bacteriol. 1998 Apr;180(7):1618-23. doi: 10.1128/JB.180.7.1618-1623.1998.
PMID: 9537355

The Oms66 (p66) protein is a Borrelia burgdorferi porin
Infect Immun. 1997 Sep;65(9):3654-61. doi: 10.1128/IAI.65.9.3654-3661.1997.
PMID: 9284133

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